Research

June 2005

June 22, 2005

Development of micro/nano fluidic devices for Amyloid Detection

Posted by David Talaga

200610191240

Fibril flow through1.jpg

June 22, 2005

June 22, 2005

Chromophore counting by discrete photobleaching events.

Posted by David Talaga

Assembly Image Steps.png

June 22, 2005

Conformational changes during ligand recognition in Glucose/Galactose Binding Protein

Posted by David Talaga

2Gbp Rendered-1

Glucose/Galactose Binding Protein (GBP) is a receptor in the chemosensory pathway of bacterial chemotaxis. GBP consists of two domains, each of which contains a beta-sheet packed between alpha-helices. The binding cleft is between the two hinged domains. Signal transduction begins in the periplasmic compartment where GBP is located. Binding of glucose or galatose by GBP causes a large amplitude conformational change that encapsulates the ligand. This allows GBP to bind to a transmembrane receptor initiating the remainder of the chemosensory pathway that regulates the bacterial flagellar motor and determines swimming behavior of the cell in response to chemical atractants or repellents

June 17, 2005

Production of a microfluidic mixer in PDMS

Posted by David Talaga

Microfluidic Mixer

This multi-channel sorting mixer was produced from a negative mold of photoresist on silicon. The mold creates an embedded design in PDMS. Subsequent bonding of the PDMS to a glass slide makes a liquid-tight seal. These fluidic devices are being used to confine bio-molecules to an observable volume by confocal microscopy so that we may watch the bio-molecules as they perform their function in real time.